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Bioanalytical Resource
(proteomics/oxidative stress markers/molecular pathology)


Director:
Stephen Barnes, Ph.D., Univ of Alabama at Birmingham

Co-Directors:
Victor Darley-Usmar, Ph.D., Univ of Alabama at Birmingham
Aimee Landar, Ph.D., Univ of Alabama at Birmingham
Satish RamachandraRao, Ph.D., University of Californina, San Diego
Daniel Sharer, Ph.D., Univ of Alabama at Birmingham


The Bioanalytical Core (Core C) serves the O’Brien Center community, both at UAB and UCSD and the extended research base by providing state-of-the-art bioenergetics, oxidative stress analysis and metabolite analysis support for AKI research. Core C provides a comprehensive resource that includes optimized protocols and technology for bioanalytical analyses of oxidative stress and cellular bioenergetics, biomarkers of AKI, post-translational modifications, and changes in small molecule biochemistry. The services involve consultation, training in experimental design, recovery of samples suitable for analysis and assay performance. New services offered include techniques to determine mitochondrial bioenergetics, LC-mass spectrometry based assays for creatinine, F2-isoprostanes and citric acid cyle intermediates. State-of-the-art nanoLC-MS methods are being developed to perform both targeted and untargeted metabolomics and exosome analysis in clinical samples and from animal models of AKI. The Core participates in education and training of investigators providing hands-on experience and scientific interchange. A recurring feature is an annual 4-day workshop for training in metabolomics (funded by NIGMS as part of the NIH Common Fund program in metabolomics).

SAMPLE PREPARATION FOR OXIDIZED FATTY ACID LC-MS


Stephen Barnes, PhD

University of Alabama at Birmingham
Birmingham, AL USA

Victor Darley-Usmar, PhD

University of Alabama at Birmingham
Birmingham, AL USA

Aimee Landar, PhD

University of Alabama at Birmingham
Birmingham, AL USA

Satish RamachandraRao, PhD

University of California, San Diego
San Diego, CA USA


Daniel Sharer, PhD

University of Alabama at Birmingham
Birmingham, AL USA